A couple of questions

Why was it important to find an enzyme that would cut the plasmid at only one site?

1. Why was it important to find an enzyme that would cut the plasmid at only one site? … If the enzymes cut at multiple spots, then you would get multiple fragments.

Why is it important that the enzyme chosen only cuts the plasmid in 1 site?

What could happen if the plasmid were cut at more than one site? (Cutting at only one site is important for controlling the variables that will be reproduced. y the restriction enzyme cut more than one site, then the plasmid might recombine with different DNAfragments.)

Why was it important to discard any enzymes that cut the plasmid at the replication site and the antibiotic resistant gene site?

Why was it important to discard any enzymes that cut the plasmid at the replication site? If the replication site was cut, it would not function. The plasmid DNA waid cease to replicate in the cell thereby not multiplying for further study.

Why was it important that they used the same restriction enzyme when cutting up each plasmid?

Restriction enzymes cut at specific sequences so the same restriction enzyme must be used because it will produce fragments with the same complementary sticky ends, making it possible for bonds to form between them.

What will happen if we use an enzyme that cuts at only one site?

They recognize and bind to specific sequences of DNA, called restriction sites. Each restriction enzyme recognizes just one or a few restriction sites. When it finds its target sequence, a restriction enzyme will make a double-stranded cut in the DNA molecule.

Why was it important to find an enzyme that would cut the plasmid at only one site what could happen if the plasmid was to cut at more than one site?

What could happen if the plasmid were cut at more than one site? You simply want to open the circular DNA so that the human DNA can be inserted into the circle. If the enzymes cut at multiple spots, then you would get multiple fragments.

Why did you discard any enzymes that cut the plasmid in the shaded plasmid replication sequence?

Discard any enzymes that cut the plasmid in the shaded plasmid replication sequence. You don't want to cut out this particular gene, because it is necessary for the bacteria to replicate itself. … The goal is to cut the DNA strand as closely as possible to the insulin gene sequence without cutting into the gene sequence.

Why is it useful for a plasmid to be a circle?

Plasmids can be copied numerous times, regardless of whether the bacterial host is replicating its own DNA, and every time a plasmid vector is replicated, so is the introduced DNA that it contains. They are circular. DNA that is circular is well suited to incorporate extra DNA sequences.