A couple of questions

Does cleavage of EcoRI result in a 5 or 3 overhang?

EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic, complementary sequence of CTTAA↓G. Other restriction enzymes, depending on their cut sites, can also leave 3' overhangs or blunt ends with no overhangs.

Which of the following restriction enzymes produce a 5 overhang?

It produces 5′overhangs (5′−C). Hind Ⅲ recognizes 5′−AAGCTT−3′ and it's complementary and produces 5′overhangs (5′−A). Hence, the correct answer is option (B).

How does EcoRI cleave?

How does EcoRI cleave its recognition site on DNA? The two protein subunits of the EcoRl restriction enzyme interact symmetrically with the recognition site on DNA, so that each subunit is in position to cleave one strand of the DNA.

Which of these enzymes create a 5 overhang after cutting DNA?

restriction enzymes Why do bacteria have restriction enzymes? Thus, it produces an overhang of 5'-AATT-3' on each end of the cut DNA. If another piece of DNA has matching overhangs (for instance, because it has also been cut by EcoRI), the overhangs can stick together by complementary base pairing.

What is a 5 prime overhang?

5' overhang- Restriction enzymes that cleave the DNA asymmetrically leave several single stranded bases. If the single-stranded bases end with a 5' phosphate, the enzyme is said to leave a 5' overhang. … If the single-stranded bases end with a 3' hydroxyl, the enzyme is said to leave a 3' overhang.

What is EcoRI restriction enzyme?

EcoRI (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system. … In molecular biology it is used as a restriction enzyme.

What do we mean when we say an enzyme has a 5 to 3 polymerase activity?

The enzymatic ability of DNA polymerase used in proof reading removes nucleotides one at a time from the 3' end of a chain. Therefore it is called 3' to 5' exonuclease activity.

Where does EcoRI cleave DNA?

GAATTC sites One popular recombinant DNA tool is the EcoRI endonuclease, which cleaves DNA at GAATTC sites and serves as a paradigm for sequence specific DNA-enzyme interactions.